Teachers & Student
Theresa Vollor - Raymond H.S.
Beth Hawkins - Madison Central H.S.
Renee Edwards - Ocean Springs H.S.
Denise Thibodeaux - Natchez Cathedral
Brady Dunaway - West Lincoln student
Jessica Franklin - Loyd Star Attendance Center
Sandra Hindsman - St. Andrew's
Lori Nelson - Ocean Springs H.S.
Edith Flores - Gulfport H.S.
Billy Sumrall - Loyd Star Attendance Center
BioRad's Bacterial Transformation (pGLO) Lab
Participants transformed a strain of E.coli using calcium chloride and heat shock which made the bacteria susceptible to accepting a foreign plasmid. The genetically engineered plasmid consisted of the glowing green jellyfish gene, an antibiotic resistant gene, and a regulator gene for arabinose (sugar). If the transformation was successful, the LB/amp/ara plate will contain bacteria that will glow in the presence of UV light. Bacteria growing on an ampicillin plate took up the plasmid but will not glow if arabinose is not present in the agar plate. GREAT lab for demonstrating the effects of a regulator gene.
-pGLO = no plasmid
+pGLO = plasmid introduced to bacteria
Under UV light.
Regular light
UV light
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Teachers also got behind-the-scenes experience in preparing and conducting BioRad's Crime Scene Lab.
The Second Meeting was at William Carey University in Hattiesburg, MS, hosted by Dr. Tyler Hodges - Sept. 21, 2013
Wolbachia Results
Gel 1 - Ran 20 minutes (30 cycles)
Lane 2 - Carpenter Ant (Ocean Springs) - infected
Lane 3 Flea - infected
Lane 4 - Mosquito (black - BC)
Lane 5 - Nas-
Lane 6 - Nas+
Lane 8 - Fire Ant
Lane 9 - Flea
Lane 10 - Mosquito (black - MC)
Lane 11 - Nas-
Lane 12- Nas+
Gel 2 - Ran 25 minutes (30 cycles)
Lane 1 - Ant
Lane 3 - Flea
Lane 4 - Mosquito
Lane 5 - Nas-
Lane 6 - Nas+
Lane 7 - Ant
Lane 8 - Flea
Lane 9 - Mosquito (white - MC)
Nas- and Nas+ tops popped open during PCR
