Sunday, March 2, 2014

Princeton Satellite Workshop @ Enterprise - March 1 & 15, 2014


Princeton Molecular Biology Workshop @ Enterprise



 Gel 1
 Gel 2 

 Gel 2 - Blue View Illuminator
 Gel 2 - Camera Filter
 Gel 3
 After opening the package that had been stored in freezer, I noticed a discrepancy with the plant primer volume. The volume of Plant Primer (green) shipped was not the same as GM Primer. There was about 6 microliters of plant primer instead of 15. The cap of the plant primer did not appear to be loose during shipping. I did not notice any leakage in the bag.






Sunday, September 15, 2013

Princeton Satellite Workshop @ Enterprise & William Carey University

Saturday, Sept. 14, 2013
Teachers & Student
 
Theresa Vollor - Raymond H.S.
Beth Hawkins - Madison Central H.S.
Renee Edwards - Ocean Springs H.S.
Denise Thibodeaux - Natchez Cathedral
Brady Dunaway - West Lincoln student
Jessica Franklin - Loyd Star Attendance Center
Sandra Hindsman - St. Andrew's
Lori Nelson - Ocean Springs H.S.
Edith Flores - Gulfport H.S.
Billy Sumrall - Loyd Star Attendance Center
 
BioRad's Bacterial Transformation (pGLO) Lab
Participants transformed a strain of E.coli using calcium chloride and heat shock which made the bacteria susceptible to accepting a foreign plasmid. The genetically engineered plasmid consisted of the glowing green jellyfish gene, an antibiotic resistant gene, and a regulator gene for arabinose (sugar). If the transformation was successful, the LB/amp/ara plate will contain bacteria that will glow in the presence of UV light. Bacteria growing on an ampicillin plate took up the plasmid but will not glow if arabinose is not present in the agar plate. GREAT lab for demonstrating the effects of a regulator gene.

-pGLO = no plasmid
+pGLO = plasmid introduced to bacteria
 
Under UV light.
 

Regular light
 

UV light
 



 
Teachers also got behind-the-scenes experience in preparing and conducting BioRad's Crime Scene Lab.
 


 

 
 
The Second Meeting was at William Carey University in Hattiesburg, MS, hosted by Dr. Tyler Hodges - Sept. 21, 2013



 
Wolbachia Results
 Gel 1 - Ran 20 minutes (30 cycles)
Lane 2 - Carpenter Ant (Ocean Springs) - infected
Lane 3 Flea - infected
Lane 4 - Mosquito (black - BC)
Lane 5 - Nas-
Lane 6 - Nas+
Lane 8 - Fire Ant
Lane 9 - Flea
Lane 10 - Mosquito (black - MC)
Lane 11 - Nas-
Lane 12- Nas+
Gel 2 - Ran 25 minutes (30 cycles)
 Lane 1 - Ant
Lane 3 - Flea
Lane 4 - Mosquito
Lane 5 - Nas-
Lane 6 - Nas+
Lane 7 - Ant
Lane 8 - Flea
Lane 9 - Mosquito (white - MC)
Nas- and Nas+ tops popped open during PCR

Saturday, August 24, 2013

Princeton Satellite Workshop @ USM's Gulf Coast Campus (June 6 & 7, 2013)

Long Beach, MS - A special thanks to Dr. Jennifer Walker for hosting this workshop.

TEACHERS:
Renee Edwards - Ocean Springs H.S.
Jeff Anderson - St. Stanislaus
Katrina Reno - Pascagoula Opportunity Center
Shani Bourn - Hancock H.S.
Bruce Hoyt - Gulfport H.S.
Tania Tellier-Brooks - Gulfport H.S.
Tammy McKenna - Gulfport Central Middle School
 











Sunday, March 10, 2013

Princeton Satellite Workshop @ Enterprise (March 9 & 23)


TEACHERS:
Denise Thibodeaux - Natchez Cathedral
Teri Vollor - Raymond H.S.
Angela Smith - Co-Lin Community College
Betsy Sullivan - Madison Central H.S.
Blaine Myers - USM student
Corey Gemelli - UMMC student
Jenna Wall - West Lincoln Attendance Center
Brady Dunaway - West Lincoln student
Beth Hawkins - Madison Central H.S.
Katie Hall - Brookhaven H.S.
Lori Nelson - Ocean Springs H.S.

 Sickle Cell Gel:  used overnight stain (BioRad 500x --> 1x)

Sickle Cell Gel:  BioRad 500x --> 100x

March 23: Teachers and parents extracted DNA from their favorite snacks to test for the "Bt" using primers for the CaMV 35S promoter and NOS terminator.

Notice the difference a filter can make when looking at Gel 2. Lane 1 & 2 have faint bands (Blaine's Sun Chip).


Blaine, Betsy, Beth, Lori, Jenna, Katie, Jessica, Amanda Lambert (parent), & Angela


Gel 1 - 2% Agarose SYBRsafe E-gel
    Exp. Date: 3/23/13      
Run Time: 22 minutes

Lanes:
Vol. µL
1

15
2
3
4
5
6
7
8
9
10
11
12
Student:

Food Sample:


Lori
Rouse’s
CornChips
PM


Lori
Rouse’s
CornChips
GM

Beth
Kroger’s
Org.
Corn Chips
PM
Beth
Kroger’s
Org.
Corn Chips
GM
Betsy
Frito
Corn Chip
PM
Betsy
Frito
Corn Chip
GM





Control
PM
MM
1,000bp
  700
  500

  200
  100
Amanda
Oatmeal
PM
Amanda
Oatmeal
GM
Angela
Chester’s
Corn Puff
PM
Angela
Chester’s
Corn Puff
GM

Results:
Confirmed GMO: Rouse's Brand Corn Chips, Sunchips, and Doritoes
Confirmed NON-GMO:  Kroger's Organic Corn Chips
Inconclusive: Frito Corn Chip, Chester's Corn Puff
Interesting: Cheetos Puff - band for GM but no plant band (Gel 2-Lanes 5&6)
 
Gel 2 - Using UV filter

Lanes:
Vol. µL
1
15
2
3
4
5
6
7
8
9
10
11
12
Student:
Food Sample:

Blaine
SunChip
PM


Blaine
SunChip
GM

Jenna
Doritoes
PM

Jenna
Doritoes
GM

Katie
Cheetoes
Puff
PM

Katie
Cheetoes
Puff
GM
dH2O
MM
Control GM
dH2O
dH2O
dH2O





Data Sheet

Friday, March 1, 2013

BioRad GMO Investigator Kit (#166-2500)

 Feb. 26 - March 1:   Biomed students tested favorite snacks for being genetically modified. Using primers for the Cauliflower Mosic Virus 35S promoter (203 bp) and Chloroplast component gene (455 bp), students were able to successfully purify plant DNA and determine the presence of the "Bt" gene.






Gel 1

Gel 2