Princeton Satellite Workshop @ Enterprise (March 9 & 23)

TEACHERS:

Denise Thibodeaux - Natchez Cathedral

Teri Vollor - Raymond H.S.

Angela Smith - Co-Lin Community College

Betsy Sullivan - Madison Central H.S.

Blaine Myers - USM student

Corey Gemelli - UMMC student

Jenna Wall - West Lincoln Attendance Center

Brady Dunaway - West Lincoln student

Beth Hawkins - Madison Central H.S.

Katie Hall - Brookhaven H.S.

Lori Nelson - Ocean Springs H.S.

 Sickle Cell Gel:  used overnight stain (BioRad 500x --> 1x)

Sickle Cell Gel:  BioRad 500x --> 100x

March 23: Teachers and parents extracted DNA from their favorite snacks to test for the "Bt" using primers for the CaMV 35S promoter and NOS terminator.

Notice the difference a filter can make when looking at Gel 2. Lane 1 & 2 have faint bands (Blaine's Sun Chip).

Blaine, Betsy, Beth, Lori, Jenna, Katie, Jessica, Amanda Lambert (parent), & Angela




Gel 1 - 2% Agarose SYBRsafe E-gel
    Exp. Date: 3/23/13      
Run Time: 22 minutes

Lanes: Vol. µL	1 15	2	3	4	5	6	7	8	9	10	11	12
Student: Food Sample:	Lori Rouse’s CornChips PM	Lori Rouse’s CornChips GM	Beth Kroger’s Org. Corn Chips PM	Beth Kroger’s Org. Corn Chips GM	Betsy Frito Corn Chip PM	Betsy Frito Corn Chip GM	Control PM	MM 1,000bp   700   500   200   100	Amanda Oatmeal PM	Amanda Oatmeal GM	Angela Chester’s Corn Puff PM	Angela Chester’s Corn Puff GM

Results:
Confirmed GMO: Rouse's Brand Corn Chips, Sunchips, and Doritoes
Confirmed NON-GMO:  Kroger's Organic Corn Chips
Inconclusive: Frito Corn Chip, Chester's Corn Puff
Interesting: Cheetos Puff - band for GM but no plant band (Gel 2-Lanes 5&6)

Lanes:

Vol. µL

1

15

2

3

4

5

6

7

8

9

10

11

12

Student:

Food Sample:

Lori

Rouse’s

CornChips

PM

Lori

Rouse’s

CornChips

GM

Beth

Kroger’s

Org.

Corn Chips

PM

Beth

Kroger’s

Org.

Corn Chips

GM

Betsy

Frito

Corn Chip

PM

Betsy

Frito

Corn Chip

GM

Control

PM

MM

1,000bp

  700

  500

  200

  100

Amanda

Oatmeal

PM

Amanda

Oatmeal

GM

Angela

Chester’s

Corn Puff

PM

Angela

Chester’s

Corn Puff

GM

 

Gel 2 - Using UV filter

Lanes: Vol. µL	1 15	2	3	4	5	6	7	8	9	10	11	12
Student: Food Sample:	Blaine SunChip PM	Blaine SunChip GM	Jenna Doritoes PM	Jenna Doritoes GM	Katie Cheetoes Puff PM	Katie Cheetoes Puff GM	dH2O	MM	Control GM	dH2O	dH2O	dH2O

Data Sheet

BioRad GMO Investigator Kit (#166-2500)

 Feb. 26 - March 1:   Biomed students tested favorite snacks for being genetically modified. Using primers for the Cauliflower Mosic Virus 35S promoter (203 bp) and Chloroplast component gene (455 bp), students were able to successfully purify plant DNA and determine the presence of the "Bt" gene.

Gel 1

Gel 2